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authentikinetm human vegf elisa kit  (Proteintech)


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    Proteintech authentikinetm human vegf elisa kit
    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of <t>VEGF</t> expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
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    Images

    1) Product Images from "KY216-tubulin complex captures VASH2 to inhibit NSCLC metastasis"

    Article Title: KY216-tubulin complex captures VASH2 to inhibit NSCLC metastasis

    Journal: Nature Communications

    doi: 10.1038/s41467-025-66817-2

    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of VEGF expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
    Figure Legend Snippet: a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of VEGF expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.

    Techniques Used: Confocal Microscopy, Labeling, Staining, Expressing, Fluorescence, Flow Cytometry, Comparison, Western Blot



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    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of <t>VEGF</t> expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
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    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of <t>VEGF</t> expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
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    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of <t>VEGF</t> expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
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    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of <t>VEGF</t> expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.
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    Image Search Results


    a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of VEGF expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: KY216-tubulin complex captures VASH2 to inhibit NSCLC metastasis

    doi: 10.1038/s41467-025-66817-2

    Figure Lengend Snippet: a Effects of KY216 on cellular microtubule networks (independent biological replicates n = 3). NCI-H460 cells were exposed to varying concentrations of KY216 for 48 h. Confocal microscopy was used to capture images of microtubules (labeled green with anti-α-tubulin antibody) and nuclei (stained with DAPI and labeled blue). Scale bar: 20 μm. b The formation of NCI-H460, A549, and 95-D cell colonies was significantly affected by different concentrations of KY216 (independant biological replicates n = 3). c Fluorescent expression of EDU+ cells was observed in NCI-H460 and 95-D cells. The EDU+ cells were labeled with green fluorescence, while the cell nuclei were stained with DAPI to show blue fluorescence (independent biological replicates n = 3). Scale bar: 100 μm. The images were acquired using the ImageXpress Micro® system. d , e Flow cytometry reveals that KY216 induces G2/M cycle arrest in NCI-H460 and 95-D cells ( d ), with significance indicated by comparison of the G2/M phase proportion in each group. Corresponding Western blot analysis is shown in ( e ) (independent biological replicates n = 3). f Conditioned medium from NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 inhibited the formation of capillary-like tubular networks in HUVECs. Representative images are shown (independent biological replicates n = 3). Scale bar: 50 μm. g Determination of VEGF expression levels in supernatants of NCI-H460 and 95-D cells treated with 0, 10, 20, and 30 nM KY216 (independent biological replicates n = 3). All quantitative data are shown as means ± SEM. Values shown are P values and were calculated using the one-way ANOVA with Tukey’s comparisons test in GraphPad Prism 9.5.0 ( b – g ). Source data are provided as a Source Data file.

    Article Snippet: The secretion of VEGF was measured using the AuthentiKineTM Human VEGF ELISA Kit (KE00216, Proteintech, China) according to the manufacturer’s guidelines.

    Techniques: Confocal Microscopy, Labeling, Staining, Expressing, Fluorescence, Flow Cytometry, Comparison, Western Blot